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Test Code LMW-SPECTRUM Low Molecular Weight Heparin

Additional Codes

Mayo Access code: SHO8102

Performing Laboratory

Spectrum Laboratory

Specimen Requirements

Specimen Collection: Blood

Container(s): 3.2% Na Citrate Tube (blue top)

Preferred Volume to Collect: 2.7 mL (full 2.7 mL blue tube)

Minimum Volume to Collect: 1.8 mL (full 1.8 mL blue white tube)

Collection Instructions:

  • Sample should be collected 4 hours after subcutaneous injection.

  • Specimen should be processed within 1 hour-See processing instructions below.

Specimen Processing and Transport Temperature

Processed Specimen: Platelet Poor Plasma

Centrifuge/Spin: Yes

Aliquot: Yes

 

Processing Instructions:

  • Double spin plasma to create platelet poor sample.
  • Separate plasma into 1 – 2 aliquots, minimum of 0.5 mL each.
  • Freeze aliquots in plastic transport tubes immediately.

Transport Temperature:

  • Ambient (up to 1 hour in whole blood state)
  • Frozen (plasma only >1 hour)

Specimen Stability

Ambient: 1 Hour

Frozen: Aliquot and freeze platelet poor plasma within 1 hour of collection.

Laboratory Retention: 24 hours

Rejection Criteria: Hemolysis

Methodology

Chromogenic Based Assay

Reference Values

The therapeutic range for anticoagulation with LMW heparin has been reported to be 0.5-1.0 U/ml and the prophylactic range as 0.2-0.4 U/ml when measured by the anti-Xa method, with samples drawn 3-4 hours after SQ administration of either dalteparin or enoxaparin.  

Test Classification and CPT Coding

CPT Code: 85520